Yes! You have two options. 

If you want to use Uproad exclusively to pay tolls:

If you no longer want to use your transponder or tag to pay tolls and exclusively use Uproad for all your needs, you will need to remove the transponder or toll tag from your vehicle's windshield. You should also remove your vehicle from your toll agency account as well. 

If you want to use Uproad only on certain toll roads:

If you want to use a transponder or toll tag in your primary driving area, you can still pay tolls with Uproad in other areas, such as when you travel outside your tolling agency’s territory.

To do this, you will keep your transponder/toll tag in your vehicle, and also activate your vehicle with Uproad. 

Inside your toll agency territory, your transponder/toll tag will cover toll costs. If you have Toll Alert turned on, you can use Uproad to track these toll charges. Your Uproad payment method will not be charged. 

When you drive on toll roads outside your territory, Uproad will become your primary toll payment method. Check our coverage map to see which toll roads Uproad includes.